THE RELATIONSHIP BETWEEN THE LEVEL OF CORTICOSTERONE AND DEHYDROEPIANDROSTERONE SULFATE IN THE BLOOD SERUM OF RATS OF DIFFERENT AGE AND SEX

  • V. V. Mizin Oles Honchar Dnipro National University
  • V. P. Lyashenko Oles Honchar Dnipro National University
  • S. M. Lukashov Medical and diagnostic scientific-consultative center “Headache”
Keywords: corticosterone, dehydroepiandrosterone sulfate, male rats, female rats, age groups

Abstract

The humoral system plays an important role in the regulation of all life processes of the body. Steroid hormones perform a variety of functions and are sensitive to the effects of internal and external factors. Therefore, these hormones are used as biomarkers of pathological and age-related changes. The research of age-related changes in the humoral system of regulation attracts particular interest. It is still important to study the levels of steroid hormones and the ratio of corticosterone / DHEA-S as a biomarker of age involution. The aim of our work was to establish the relationship between the level of corticosterone and dehydroepiandrosterone sulfate in the blood serum of rats of different age and sex.

The experiments were carried out on non-linear, nonbreeding white rat males and females. Which were divided into 8 groups: group I ‒ males of the juvenile age of the sexually mature period, (n = 15); II group ‒ females of the juvenile age of the sexually mature period, (n = 15); III group ‒ males of young age of the reproductive period, (n = 14); IV group ‒ females of a young age of the reproductive period, (n = 14); V group ‒ males of mature age of the reproductive period, (n = 14); VI group - females of the mature age of the reproductive period, (n = 14); VII group ‒ males of the premature age of the period of aging changes, (n = 14); VIII group - females of the premature age of the period of aging changes, (n = 14). The level of hormones was determined by electro-chemiluminescent method on the Roche Elecsys 2010 analyzer.

We analyzed the results and found that in males and females of juvenile age a significantly high level of corticosterone relative to rats of other age groups. The lowest level of corticosterone was contained in the serum of rats of mature age – 44,4 ± 4,8 nmol / l. In pre-adult age, in males, the level of coryrosterone rose to 117,6 ± 2,6 nmol / l, and in females to 174,32 ± 3,6 nmol / l. The level of DHEA-S in males is significantly high in the juvenile group – 326,55 ± 13,73 nmol / l, and in adulthood it is lower by 91,6 %. In the pre-teen age of males, the level of the hormone significantly increased. A high positive correlation was found between age-related changes in these hormones (r = 0,81, p < 0,05) in male rats. A significantly high level of DHEA-S in females of young age of reproductive period – 450,58 ± 14,7 nmol / l, and in adulthood the indicator is 5,8 times lower. In pre-teen age, the level of the hormone increased. The correlation between changes in hormones is positive moderate (r = 0,48, p < 0,05). The ratio of corticosterone / DHEA-S exceeded one in males of mature age and in females of juvenile age.

The results obtained may indicate the presence of cyclic activity in the synthesis of steroid hormones in rats of different ages and sex. In males from juvenile to adulthood, the synthesis of corticosterone and DHEA-S was reliably reduced, and in the age of pre-activation, the synthesis of these hormones was activated again. Similar changes occurred in females with corticosterone synthesis, but the level of DHEA-S changed wavyly with a peak at a young age.

References

ЛІТЕРАТУРА
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7. Low Serum DHEA-S Predicts Increased Fracture Risk in Older Men: The MrOS Sweden Study / Ohlsson C. et all. Journal of Bone Miner Research. 2017. Vol. 32, № 8. P. 1607-1614.
8. Sánchez-Guijo A., Neunzig J., Gerber A., Oji V. Role of steroid sulfatase in steroid homeostasis and characterization of the sulfated steroid pathway: Evidence from steroid sulfatase deficiency. Molecular and Cellular Endocrinology. 2016. № 437. P. 142-153.
9. Kamin H. S., Kertes D. A. Cortisol and DHEA in development and psychopathology. Hormones and Behavior. 2017. № 89. P. 69-85.
10. Walking training and cortisol to DHEA-S ratio in postmenopause: An intervention study / Di Blasio A. et all. Women Health. 2017. № 1. P. 1-16.
11. Jina R. O., Masona S., Mellon S. H. Cortisol/DHEA ratio and hippocampal volume: A pilot study in major depression and healthy controls. Psychoneuroendocrinology. 2016. № 72. P. 139-146.
12. Dehydroepiandrosterone alters vitamin E status and prevents lipid peroxidation in vitamin E-deficient rats / Miyazaki H. et all. Journal of Clinical Biochemistry and Nutrition. 2016. Vol. 58, № 3. P. 223-231.
13. Beishuizen A., Groeneveld A. B. J. Dehydroepiandrosterone (DHEA) and its Sulfate (DHEAS) in Critical Illness. Intensive Care Medicine. 2003. № 1. P. 891-900.
14. Mechanism of action of anti-aging DHEA-S and the replacement of DHEA-S / Nawata H. et all. Steroids. 2008. № 73 (2). P. 209-215.
How to Cite
Mizin, V. V., Lyashenko, V. P., & Lukashov, S. M. (1). THE RELATIONSHIP BETWEEN THE LEVEL OF CORTICOSTERONE AND DEHYDROEPIANDROSTERONE SULFATE IN THE BLOOD SERUM OF RATS OF DIFFERENT AGE AND SEX. Bulletin of Zaporizhzhia National University. Biological Sciences, (2), 67-74. Retrieved from http://journalsofznu.zp.ua/index.php/biology/article/view/98