OBLIGATNO-ANAEROBIC AND FACULTATIVE-ANAEROBIC MICROORGANISMS AT AN ACUTE NECROTIC PANCREATITIS

  • V. V. Potochilova Communal establishment of the Kyiv regional soviet is the “Kyiv regional clinical hospital”
  • V. G. Voicehоvskii National Bogomolets Medical University
Keywords: acute necrotic pancreatitis, obligate anaerobic microorganisms, facultative anaerobic microorganisms

Abstract

Recently, more and more data appear that confirm that in the etiology of purulent necrotic pancreatitis an important place, along with aerobic and facultative anaerobic microorganisms, occupy obligate anaerobic bacteria.

The main clinical and morphological forms of pancreatic infection are infected pancreatic necrosis and pancreatic abscesses and suppuration of false (post-necrotic) cysts of the pancreas.

Recently, the number of patients with inflammatory diseases of the pancreas has increased, especially in the elderly with complicated forms of pancreatitis, and the absence of a single pathogenetically determined treatment method does not contribute to a decrease in the high mortality rate for purulent-septic postoperative complications.

The obtained results confirm the necessity of comprehensive studies on the establishment of etiology, the study of pathogenesis, and the improvement of treatment tactics in the purulent-inflammatory diseases of the pancreas.

 

86 patients diagnosed with acute necrotizing pancreatitis were hospitalized in the surgical department of the Kyiv Oblast Clinical Hospital of Kyiv Oblast during the period from 2011 to 2016. Of these, 51 male patients (59,31 %) and 35 female (40,69 %).

Purulent content from the pancreas was taken from each patient, both during endoscopic examination and during surgical interventions and with sanation of the abdominal cavity.

To isolate anaerobic microorganisms, a fresh or pre-cooked thioglycolic medium with resazurin was used. The thioglycolic medium, prepared in advance, was regenerated in a water bath for 45 minutes at a temperature of 100 °C.

To select the facultative anaerobic microorganisms, selective and elective nutrient media were used and the material was also scattered using the Gold method. At the same time, the material was sown in a sugar broth, from which every 24 hours they moved to a dense nutrient medium.

In the examination of 86 patients with purulent content, 254 strains of microorganisms were isolated. Of these, 151 strains are isolated from the material obtained during endoscopic examination. 103 surgical strains of microorganisms were identified during surgical interventions and during sanation of the abdominal cavity.

The number of bonded-anaerobic bacteria was higher during endoscopic examination than during surgical interventions and sanation of the abdominal cavity.

This may be due to the fact that bonded-anaerobic microorganisms die when oxygen enters the salivary bag after surgical intervention, or because patients during the surgical intervention stage have already been impulsively receiving aggressive antibiotic therapy.

Evaluating the data obtained, we can conclude that the microorganisms that are presented in acute necrotic pancreatitis are variable. Associations of microorganisms were allocated more often. Less isolated monocultures of microorganisms. In patients with acute necrotic pancreatitis, 254 clinical strains of microorganisms, which were 21 species, were studied. The obtained types of microorganisms are the main causative agents of acute necrotic pancreatitis in the examined patients.

In the study of pus during and after surgical interventions, it has been established that the microflora of purulent necrotic pancreatitis is more often represented by the microorganisms of the families Enterobacteriaceae, Pseudomonadaceae and Clostridiaceae. Therefore, we can assume that pancreatic contamination occurs from the gastrointestinal tract, in violation of the barrier functions of the intestinal mucosa as a result of translocation.

Conducting microbiological research in dynamics showed that there is a change in the qualitative composition of the microflora purulent content obtained during and after surgical interventions, compared with the microflora obtained during the endoscopic examination.

In our study, 254 cultures of facultative anaerobic and obligate anaerobic microorganisms were analyzed, which included 10 families, and 2 cultures of fungi of the genus Candida.

It was established that microflora in acute necrotic pancreatitis is not stable, but the types of microorganisms isolated are the main causative agents of purulent complications of acute pancreatitis in the examined patients.

Bacterial translocation from the lumen of the intestine, due to the violation of the barrier function of the intestinal wall, is considered to be the main mechanism of the onset of purulent-septic complications of acute necrotic pancreatitis, which is confirmed by the results obtained.

References

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How to Cite
Potochilova, V. V., & VoicehоvskiiV. G. (1). OBLIGATNO-ANAEROBIC AND FACULTATIVE-ANAEROBIC MICROORGANISMS AT AN ACUTE NECROTIC PANCREATITIS. Bulletin of Zaporizhzhia National University. Biological Sciences, (2), 112-118. Retrieved from http://journalsofznu.zp.ua/index.php/biology/article/view/104