DYNAMICS OF ACTIVITY OF LIPID PEROXIDATION PROCESSES AND SYSTEM OF PROOXIDANT-ANTIOXIDANT PROTECTION IN THE LIVER OF RATS IN CONDITIONS OF OXIDATIVE STRESS BY CORRECTION WITH ANTIOXIDANT COMPLEX

  • Ya. V. Diorditsa Kherson State University
  • A. L. Garkovich Odessa National Academy of Food Technologies
  • V. I. Yezikov Kherson State University
Keywords: hydrazine sulfate, diene conjugates, malonic dialdehyde, superoxide dismutase, catalase, quercetin, «Triovit», α-lipoic acid

Abstract

Excessive environmental load, taking medicine, alcohol, drugs, food additives, using of pesticides and herbicides, household chemicals lead to structural and functional disorders of hepatocytes. The liver is an important gland of the body participated in the elimination of a number of endogenous toxic metabolites and exogenous xenobiotics, contributing to their excretion from the body. The complex effect of xenobiotics causes intensification of lipid peroxidation processes and destabilization of antioxidant defense of the organism. In the normal physiological state of the body, the rate of lipid peroxidation processes and the activity of the antioxidant system are in balance. When the balance move to the side of the lipid peroxidation processes - oxidative stress is developed, which is a predecessor for the development of pathologies of different origins. Products of lipid peroxidation processes – malonic dialdehyde (MDA) and diene conjugates (DC) are markers of tissue damage and have cytotoxic and mutagenic effects. These effects leveled with antioxidants. The article present the result of testing the influence of the antioxidant complex on the dynamics of lipid peroxidation parameters and the activity of enzymes of the prooxidant and antioxidant system of rat’s liver under conditions of oxidative stress caused by single administration of hydrazine sulfate.The experiments carried out on white laboratory rats of the Vistar line weighing 280 g, which kept under standard vivarium conditions. The rats divided into 4 groups: I group – control (n = 6); ІІ – acute toxic hepatitis, caused by a single intra-peritoneal injection of a solution of hydrazine sulfate (100 mg / kg) (n = 6); III – hepatitis + «Triovit» (50 mg / kg) and drug quercetin (20 mg / kg) (n = 6); IV – hepatit + «Triovit» (50 mg / kg) + quercetin (20 mg / kg) + lipoic acid (100 mg / kg) (n = 6). Complex of preparations the rats obtained per os immediately after the introduction of hydrazine sulfate. Animals extracted from the experiment under ethereal anesthesia, the decapitation carried out 24 hours after started of experiment.Intra-peritoneal administration hydrazine sulfate to experimental rats at a dose of 100 mg / kg causes liver damage due to increased formation of active forms of oxygen and activation of free radical oxidation of biomembranes of hepatocytes. It is confirmed by the growth of lipid peroxide oxidation products – malon dialdehyde and diene conjugates, decrease in the activity of antioxidant enzymes. In animals, which not treated with antioxidants, the level of primary lipid peroxidation products – DC increased by 78.05 % and secondary products of lipid peroxidation – MDA – by 19 % compared to control one. We used antioxidant complexes complex for normalization indexes of the lipid peroxidation. When we used for the treatment the antioxidant complex which containing quercetin and «Triovit», which includes vitamins A, E, C and microelement Selenium, we observe lowering the level of DK by 45 % and MDA by 8 % compared to a non-treated rats. When we use antioxidant complex, that including «Triovit», quercetin and α-lipoiс acid the level of DC slightly lowered, and MDA increased by 44.6 % compared to non-treated animals with antioxidants. The activation of lipid peroxidation led to a change in the activity of the main antioxidant enzymes - superoxide dismutase (SOD) and catalase. Introduction the xenobiotic decreased the level of the SOD by 20.43 % compared with intact. Probable changes in the catalase index we are not noted. With quercetin and «Triovit” correction, SOD indexes grew by 5.3 % compared to the non-treated group, but these indexes were 14.4 % lower than intact rats. The catalase index have not reliable change. Treatment of «Тriovit», quercetin and lipoiс acid decreased the level of SOD by 16.5 % compared with animals without antioxidant treatment and by 22.7 % сcompared to the rats which treated with «Тriovit» and quercetin. Using the complex of antioxidant, which includes «Triovit» and quercetin in the conditions of oxidative stress promote to the normalization of the processes of lipid peroxidation, returning to normal the index of MDA and DK, promote restoration of the SOD index.

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Published
2018-07-03
How to Cite
Diorditsa, Y. V., Garkovich, A. L., & Yezikov, V. I. (2018). DYNAMICS OF ACTIVITY OF LIPID PEROXIDATION PROCESSES AND SYSTEM OF PROOXIDANT-ANTIOXIDANT PROTECTION IN THE LIVER OF RATS IN CONDITIONS OF OXIDATIVE STRESS BY CORRECTION WITH ANTIOXIDANT COMPLEX. Bulletin of Zaporizhzhia National University. Biological Sciences, (1), 58-63. https://doi.org/10.26661/2410-0943-2018-1-08