AGE-RELATED CHANGES OF OPEN FIELD BEHAVIORAL REACTIONS OF MALE AND FEMALE RATS

  • V. V. Mukvych Oles Honchar Dnipro National University
  • V. P. Liashenko Oles Honchar Dnipro National University
  • S. M. Lukashov Medical-diagnostic scientific-consulting Center “Headache”
Keywords: behavioral reactions, male and female rats, juvenile puberty period, young age of the reproductive period, mature age of the reproductive period, presenile age of the pronounced senile changes period, open field test

Abstract

The Paper presents an estimation of age- and sex-related changes of open field behavioral reactions of male and female rats under normal conditions.It is known that to prevent the effects of cyclic changes in hormonal regulation of females, males are most often used in studies. In the study we used both male and female rats of different age categories to detect the sexual characteristics of behavioral reactions at different age stages of ontogenesis. The study was conducted on 120 non-linear outbred white male and female rats. The animals were distributed into groups using the classification of age groups of laboratory animals by Zapadniuk I. P. According to the classification male rats of 2,5 months belong to the juvenile puberty period (I group), of eight months – to the young age of the reproductive period (II group), of fourteen months – to the mature age of the reproductive period (ІІІ group), of 21 months – presenile age of the pronounced senile changes period (IV group). The main components of the open fieldratsbehavior were analyzed, which in turn characterize one or another type of animals activity. We directly traced the state and age changes in the motor (crossing the squares along the periphery), research (crossing the squares in the center, number of pillars and examined holes), vegetative (number of urinations and defecations) activities, and also analyzed animal anxiety level (number of fading and grooming).

It is established that there is a probable decrease in the number of squares crossed along the periphery in female and male rats of four age groups, indicating a significant change in their motor activity with age. Female rats of different age groups have higher rates of horizontal motor activity and are characterized by their sharp decrease with age compared to male rats. The analysis of age-related changes in the research activity indexes allows us to determine the probable decrease in the number of squares crossed in the center, the pillars and the examined holes in female and male rats of different age groups. Fading and grooming, as indicators of ratsanxiety are likely to increase with age and the peak of their increase falls on presenile rats. Vegetative indices of the studied male and female rats of all age periods were characterized by almost similar gradual increases in the number of urinations and defecations. Presenile female rats had a sharp increase in the number of defecations by 4 times and males of the corresponding age - a probable increase in the number of urinationsby 9 times compared to juvenile rats of the same sex. Consequently, the studyresults made it possible to establish both age- and sex-related changes of behavioral reactions of male and female rats at different age stages of their ontogenesis.

References

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How to Cite
Mukvych, V. V., Liashenko, V. P., & Lukashov, S. M. (1). AGE-RELATED CHANGES OF OPEN FIELD BEHAVIORAL REACTIONS OF MALE AND FEMALE RATS. Bulletin of Zaporizhzhia National University. Biological Sciences, (2), 75-84. Retrieved from http://journalsofznu.zp.ua/index.php/biology/article/view/99