INFLUENCE OF MELANINE ON THE MORPHOFUNCTIONAL STATE OF RAT SKIN WITH EXPERIMENTAL WOUNDS

  • O. V. Taburets Taras Shevchenko National University of Kiev
  • V. V. Vereschaka Taras Shevchenko National University of Kiev
  • T. V. Beregova Taras Shevchenko National University of Kiev
  • L. I. Ostapchenko Taras Shevchenko National University of Kiev
Keywords: cut wound, chemical burn, melanin, skin

Abstract

Wound healing is a complex and dynamic process of replacing devitalized and missing cellular structures and tissue layers. We have previously shown that melanin, producer of which is the Antarctic black yeast fungi Pseudonadsoniella brunea (Nadsoniella nigra sp. X-1), sown with samples of vertical cliffs of the island Galindez (Ukrainian Antarctic Station “Akademik Vernadsky”) has expressed cytoprotective effect, promoted rapid wound healing of various ethiology and can be offered as a new dermatropic drug. We have created a new pharmacological composition which includes 0,1 % melanin, dissolved in 0,5 % Carbopol.

Experiments conducted in accordance with international principles of the European Convention for the Protection of vertebrate animals used for experimental and other scientific purposes, according to the Law of Ukraine of 21.02.2006 № 3447-IV “On protection of animals from cruelty”.

Research was conducted on white laboratory female rats weighing 200-250 g, which were divided into four groups. In each model animals experimental skin wounds without drugs were used as a control (first group). Wounds of rats of second group were treated only with 0,5 % carbopol (universal solvent drugs to make them gel-like consistency, Carbopol 980”). Animals of third group got 0,1 % melanin (produced by Antarctic black yeast-like fungi Nadsoniella nigra, strain X1-M,and received by us microbiologically) dissolved in 0,5 % carbopol for wounds’ healing. Animals of fourth group without experimental skin wounds were used as a intact animals. Before the experiment, the rats were kept in quarantine and were marked by given them notches on ears. When animas were injured they were anesthetized by sodium thiopental (BiochemieGmbH/Austria), at a dosage of 50 mg/kg. Before the experiment epilation was performed in the shoulder-blade area. Model of full-thickness skin wound. Plate wounds are reproduced on epilated skin in anesthetized rats. To do this, skin is cut using surgical scalpel and forceps, 1 × 1sm2.

Chemical skin burns were caused by the introduction of 0,1 ml CaCl2 The attention was payed to the standardization of wounds received, the size of which didn’t exceed 400 mm2. On 4-5 days necrotomy of the affected area was performed and then treatment of wounds started until healing

Treatment begins immediately after wounds reproduction until healing.

Histological study was conducted using standard histological methods.

When analyzing the histological sections of the skin of rats, we can visually observe the efficiency of the regenerative processes of the restoration of the epidermis, dermis and hypodermis due to the growth and replacement of deformed and necrotic areas with collagen fibers. Accordingly, on the 14th day of the studies in the experimental group of animals, which were applied to the wound wound with the melanin-based composition, the mature connective tissue predominated, the leukocyte infiltration was already poorly expressed, and the epithelization of the wound was well expressed in the layers of the epidermis. In control and group II animals, leukocyte infiltration and granulocyte infiltration was moderately pronounced, a large number of neutrophilic leukocytes, eosinophils and macrophages were observed, both in the purulent part of the skin at the site of necrosis and at the border with it. The process of regeneration of the epidermis was weakly expressed in contrast to the indices of group III.

Thus, during the experimental researches, the establishment of a “coarse scar” in the control group and in the second group of rats with the subsequent formation of a purulent site at the site of skin necrosis was determined by the long-term inflammatory process in the injury zone, which does not occur in the treatment of the pharmacological composition developed by us on the basis of Melanin Melanin, with its external application, modulates the process of inflammation, which leads to restoration of the structure of the skin with its mechanical damage. Therefore, the melanin-based composition is an effective dermatotropic agent for the treatment of wounds of skin of various genesis

In future studies, it is planned to conduct molecular and biochemical studies in blood serum and skin homogenate in animals with cutaneous wounds, without treatment and in conditions of melanin correction, and also compare the effective of treatment of cutaneous damage with melanin and with other dermatotropic-mediated preparations.

References

ЛІТЕРАТУРА
1. Barrett S. Wound-bed preparation: a vital step in the healing process. British journal of nursing. 2017. Vol. 26, №. 12. P. 24-31.
2. Christine M. Jones, Alexis T. Rothermel, Donald R. Mackay. Evidence-based medicine: wound management. Plastic and reconstructive surgery. 2017.Vol. 40, №. 1. P. 201-216.
3. Fahimi S. et al. Wound healing activity of a traditionally used poly herbal product in a burn wound model in rats. Iranian Red Crescent Medical Journal. 2015. Vol. 17, №. 9. P. 1-8.
4. Зимон А. Д., Лещенко А. Д. Коллоидная химия: Химия. Москва, 1995. 326 с.
5. Taburets O. V. et all. The Effect of “Melanin-Gel” on the Wound Healing. RJPBCS. 2016. Vol. 7, № 3. P. 2031-2038.
6. Dranitsina A. S., Taburets O. V., Dvorshchenko K. O. et all. TGFB 1, PTGS 2 Genes Expression during Dynamics of Wound Healing and with the Treatment of Melanin. RJPBCS. 2017. Vol. 8, №1. P. 2014-23.
7. Taburets O. V., Grinchenko O. O., Dvorschenko K. O. et all. Influence of the melanin on the state of prooxidant-antioxidant homeostasis in blood serum at the rats with the full-thickness skin wound. Bulletin of problems biology and medicine. 2017. Vol.1 (135). P. 191-96.
8. Перший національний конгрес з біоетики. Еженедельник аптека. 2001. № 308 (37) (від 24.09.2001).
9. Bilyayeva O., Neshta V. V., Golub A. et all. Effects of Sea Silon wound healing in the rat. Journal of wound care. 2014. Vol. 23, №8. Р.140–146.
10. Schäfer M., Werner S. Oxidative stress in normal and impaired wound repair. Pharmacological Research. 2008. Vol. 58. P. 165-171.
11. Mescher A. Junqueira’s basic histology: text and atlas. McGraw-Hill; 12 ed., 2009.480 p.
12. Саркисов Д. С., Перов Ю. Л. Микроскопическая техника: Руководство. Москва: Медицина, 1996. 544 с.

REFERENCES
1. Barrett S. Wound-bed preparation: a vital step in the healing process. British journal of nursing. 2017. Vol. 26, №. 12. P. 24-31.
2. Christine M. Jones, Alexis T. Rothermel, Donald R. Mackay. Evidence-based medicine: wound management. Plastic and reconstructive surgery. 2017. Vol. 40, №. 1. P. 201-216.
3. Fahimi S. et all. Wound healing activity of a traditionally used poly herbal product in a burn wound model in rats. Iranian Red Crescent Medical Journal. 2015. Vol. 17, №. 9. P. 1-8
4. Zimon A. D., Leshenko A. D. Koloidnaya khimiya: Khimiya. Moskva. 1995. 326 s.
5. Taburets O. V. et all. The Effect of “Melanin-Gel” on the Wound Healing. RJPBCS.2016.Vol. 7, № 3.
P. 2031-2038.
6. Dranitsina A.S., Taburets O. V., Dvorshchenko K. O. et all. TGFB 1, PTGS 2 Genes Expression during Dynamics of Wound Healing and with the Treatment of Melanin. RJPBCS. 2017. Vol. 8, №1. P. 2014–23.
7. Taburets O. V., Grinchenko O. O., Dvorschenko K. O. et all. Influence of the melanin on the state of prooxidant-antioxidant homeostasis in blood serum at the rats with the full-thickness skin wound. Bulletin of problems biology and medicine. 2017. Vol.1 (135). P. 191-96.
8. Pershyy natsіonalnyy kongres z bіoetyky. Ezhenedelnik apteka. 2001. № 308 (37) (vіd 24.09.2001).
9. Bilyayeva O., Neshta V. V., Golub A. et all. Effects of Sea Silon wound healing in the rat. Journal of wound care. 2014. Vol. 23, №8. Р.140-146.
10. Schäfer M., Werner S. Oxidative stress in normal and impaired wound repair. Pharmacological Research. 2008. Vol. 58. P. 165-171.
11. Mescher A. Junqueira’s basic histology: text and atlas. McGraw-Hill; 12 ed., 2009. 480 p.
12. Sarkisov D. S., Perova Yu. L. Mikroskopicheskaya tekhnika: Rukovodstvo. Moskva: Meditsina, 1996. 544 s.
How to Cite
Taburets, O. V., Vereschaka, V. V., Beregova, T. V., & Ostapchenko, L. I. (1). INFLUENCE OF MELANINE ON THE MORPHOFUNCTIONAL STATE OF RAT SKIN WITH EXPERIMENTAL WOUNDS. Bulletin of Zaporizhzhia National University. Biological Sciences, (2), 93-98. Retrieved from http://journalsofznu.zp.ua/index.php/biology/article/view/101