METHODS OF ESTIMATION OF INTENSITIVITY OF PIGMENT FORMATION OF MICROORGANISMS AND THEIR IDENTIFICATION

  • K. S. Krupey Zaporizhzhya National University
Keywords: microorganisms, identification, pigments, computer programs

Abstract

Previous studies on pigmentation bacteria and yeast have shown that they can be used for bioindication of compounds of heavy metals (HM) in the environment. The intensity of the color of pigments allows us to make a visual assessment of the influence of environmental factors on the cells of microorganisms. For the quantitative expression of the difference in the intensity of the color of the pigments between the control and experimental samples of microorganisms using computer software AdobePhotoshop and CIEDE2000. In this paper, an attempt was made to evaluate the intensity of pigmentation of microorganisms by other methods and to develop a method for the identification of pigmented forms of microflora. Therefore, the purpose of our research was to create a table-determinant of pigment-synthesizing microorganisms using known computer programs.

For the compilation of a table-determinant of microorganisms, a digital image of samples of microorganisms culture was performed. The digital image was transferred to the computer in the AdobePhotoshop program, and they determined the colors of the colonies of microorganisms. Then, using the CIE Lab program packages, the color model values were calculated: the colors of the test samples of microorganisms compared with the control color (violet), with the values of the color model L = 16, a = 37, b = -18 were compared. It is the comparison with this color of orange and red hues that provides the greatest difference in the intensity of color between colonies of microorganisms. For example, a very slight difference in the color intensity between the studied cultures was observed for the black (L = 0, a = 0, b = 0), white (L = 99, a = 0, b = 0) and green (L = 31, a = -33, b = 22) colors. After that, the index of investigated microorganisms was compared with the indicators of earlier identified species, the species composition of pigment-synthesizing microorganisms was identified by this indicator.

The basis of our patent was the task of developing a method for identifying pigment-synthesizing microorganisms, by growing microorganisms on the nutrient medium, detecting the shades of their color in a digital form, determining the difference in the color intensity of the colonies compared with the standard, comparing the data obtained with the data of already identified microorganisms will increase the speed and visibility of the method, simplify the process of identification of microorganisms. Also, this method will provide the opportunity to perform other stages of identification of the species belonging to microorganisms, and to create an appropriate database of pigment-synthesizing microorganisms according to these indicators.

In developing the method, we drew attention to the known method of determining the intensity of pigmentation in bacteria, which, however, does not allow to identify the species composition of microorganisms.

Even among one systematic group of microorganisms there are strains that produce different qualitative composition of pigments, as a result of which dE is very different between them. So, for spores Sporobolomyces roseus Y-1443 dE was 33,93 and for strain Sp. roseus Y-1444 dE was 10 units bigger (43,55).

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Published
2018-07-03
How to Cite
Krupey, K. S. (2018). METHODS OF ESTIMATION OF INTENSITIVITY OF PIGMENT FORMATION OF MICROORGANISMS AND THEIR IDENTIFICATION. Bulletin of Zaporizhzhia National University. Biological Sciences, (1), 90-96. https://doi.org/10.26661/2410-0943-2018-1-13